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KMID : 0359319800200020079
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Korean Journal of Veterinary Research
1980 Volume.20 No. 2 p.79 ~ p.84
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Isolation and Drug Sensitivity of Gram - Negative Rods from Bovine Udder Infections
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Abstract
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One hundred and eighteen cultures of Gram-negative rods isolated from cases of clinical bovine mastitis during lactation were examined for distribution of specific types, and activity of several antimicrobial agents to the isolates was determined by two-fold tube dilution method employing sterile whole milk as fluid medium.
Of the isolates, 59.2% were Escherichia coli. Most of the remaining isolates were Klebsiella pneumoniae and Enterobacter aerogenes. Most Gram-negative rods(89.8%) were isolated from acute local and chronic mastitis. The cases of peracute systemic form with a marked symptoms of toxemia were associated with Escherichia coli and Klebsiella pneumonia.
The minimal lethal concentrations (MLC) of gentamicin and oxolinic acid in sterile whole milk were 16-128 times higher than the MLC obtained in trypticase soy broth (TSB), while the MLC of ampicillin and tetracycline in milk increased 2-4 times compared with TSB.
Of the drugs tested, gentamicin was the most active antibiotics with MLC of 100㎍/㎖ in sterile whole milk against all of Gram-negative rods isolated from bovine udder infections.
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